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Figure 1b shows pulse field gel electrophoresis analysis of wild type HVS in comparison with the recombinant HVS Luc, demonstrating correct insertion of the Luc cassette into the HVS BAC. (a) A luciferase expression cassette containing the luciferase gene under the control of the SV40 promoter alongside a kanamycin resistence cassette were inserted into the HVS BAC at the unique I Ppo I restriction, to generate HVS Luc. (b) To confirm that the heterologous DNA was inserted into the correct position within the HVS BAC, a restriction digest on wild type HVS and HVS Luc was performed using I Ppo I and Age I restriction enzymes.